Bioinformatic Analysis of the Impact of MicroRNA Modulation on Signaling Pathways in CancerJetter, Lily MicroRNAsiRNABioinformaticsBioinformatic AnalysisQIAGEN IPACancerP53 signalingWNT/Beta-cateninPI3K/AKTCCND1BCL2miRNABiomedical EngineeringB SMicroRNA affects the expression of its target genes within each individual cell. Through manipulating levels of microRNA, it is possible to control the expression of target genes; however, effects can be numerous and widespread. By choosing microRNA with target genes involved in common cancer pathways, the progression and migration of cancer can be directed. This thesis focuses specifically on the microRNA strands miR-34a-5p and miR-148b-3p and their role within the development of cancer. Each microRNA was analyzed through three different canonical pathways: P53 signaling, WNT/Beta-catenin signaling and PI3K/AKT signaling. Using QIAGEN IPA, both microRNAs were analyzed through the lens of each canonical pathway to identify targets. By finding commonalities within these targets, CCND1 and BCL2 were able to be identified as promising targets to combat cancer progression. Chosen genes can then be targeted using small interfering RNA (siRNA) applications to avoid off target effects associated with microRNA.Daniel J Hayes, Thesis SupervisorMeghan Vidt, Thesis Honors Advisoropen_access2026-03-30T19:11:11Zhttps://honors.libraries.psu.edu/catalog/10346lej5199