DNA FINGERPRINTING AND GENOTYPING IN PRUNUS SEROTINA
Open Access
Author:
Devine, Daniel Joseph
Area of Honors:
Biology
Degree:
Bachelor of Science
Document Type:
Thesis
Thesis Supervisors:
John Edward Carlson, Thesis Supervisor John Edward Carlson, Thesis Supervisor Richard Cyr, Thesis Honors Advisor
Keywords:
DNA fingerprinting linkage mapping black cherry
Abstract:
The main goal of this study was to perform DNA fingerprinting and identify full siblings in a population of black cherry (Prunus serotina) seedlings, an economically and ecologically valuable hardwood tree species native to the eastern United States and invasive in Europe. Fingerprinting was performed on 600 samples from the ozone sensitive R-14 family of the black cherry. To fingerprint, SSR markers previously identified for a black cherry EST library and found to be polymorphic were used. DNA was extracted from the 600 samples and was used to perform PCR followed by genotyping. At this point in time, 2 markers (131 and 654) have been found to be polymorphic in black cherry. Once an additional 4 to 8 polymorphic markers are found, fingerprinting for the 600 samples will ensue. This data will then be used to establish linkage maps for quantitative trait loci related to ozone in black cherry.
