THE CHARACTERIZATION OF A MICROCHANNEL DEVICE USING LASER BASED MICRO-PIV

Open Access
Author:
Wang , Sicong
Area of Honors:
Bioengineering
Degree:
Bachelor of Science
Document Type:
Thesis
Thesis Supervisors:
  • Siyang Zheng, Thesis Supervisor
  • Peter J Butler, Honors Advisor
  • William O Hancock, Faculty Reader
Keywords:
  • microchannel
  • microfluidics
  • micro-PIV
  • soft lithography
  • murray law
  • circulating tumor cell
  • velocity profile
  • volumetric flow rate
  • pulastive flow
  • PDMS
Abstract:
Cancer has been one of the most deadly diseases in the world. Numerous studies have been done in this field, but still to this day, no significant breakthrough has produced viable treatment for this vicious illness, besides early surgical resection of the tumors. 90% of the cancer patients died because of metastatic disease, which is believed to propagate by the spreading of circulating tumor cells (CTCs) through vascular and lymphatic circulation. New research needed to be done to test and study the behavior of the CTCs, one of the primary sources of new tumors, in the environment of the human bloodstream. Although single channeled devices have been created to mimic the condition of human blood vessels, and their flow velocity profile has been tested, no comprehensive multi-branched device has been designed and tested. In this study, a micro-fabricated device is designed according to Murray’s law to mimic the natural changes in blood flow accompanying blood vessel branching in order understand the effects of flow rate, shear stress and branching on CTCs. Through testing using micro-PIV, the velocity profile of the device is generated and the volumetric velocities of the micro-channels are calculated. The experimental values of the volumetric velocity deviate from the theoretical values, which prove that this device needed to be revised. However, the researchers could use the existing device, calibrated with the velocity profile collected, to conduct further studies on CTCs.