transcriptome gibbon ape Hylobates agilis albibarbis Y-chromosome
Abstract:
This study was designed to identify Y-chromosome specific genes from the Hylobates agilis albibarbis gibbon. A testes tissue sample was used to create a cDNA library of the gibbon using Illumina NGS technology. This cDNA library was reconstructed de novo to create a transcriptome. The transcriptome was analyzed using two methods to identify Y-chromosome specific genes by comparison to published genes of related species, human (Homo sapiens) and chimpanzee (Pan troglodytes). Genes identified multiple times were considered truly robust for analysis, which resulted in 16 genes identified. These genes were then further analyzed for their function, depth, coverage, and selection pressure.
