Pterostilbene inhibits the growth of human non-small cell lung cancer cells by inducing cell cycle arrest

Open Access
Author:
Vuong, Ashley C
Area of Honors:
Food Science
Degree:
Bachelor of Science
Document Type:
Thesis
Thesis Supervisors:
  • Joshua D Lambert, Thesis Supervisor
  • Pamela Hankey, Honors Advisor
  • John Neil Coupland, Faculty Reader
Keywords:
  • pterostilbene
  • food science
  • cell cycle arrest
  • lung cancer
  • cancer
  • blueberries
  • resveratrol
Abstract:
Pterostilbene is a dimethylated analog of resveratrol and is commonly found in blueberries.  We tested the hypothesis that pterostilbene could inhibit the growth of non-small cell lung cancer cells in vitro.  Pterostilbene dose-dependently reduced viability of H1299 human lung cancer cells (IC50 = 17.6 μM - 49.0 μM). Time-dependent studies with 25 μM and 50 μM pterostilbene showed that this loss of viability was due to growth inhibition rather than cytotoxicity.  Cell cycle analysis showed that pterostilbene induced G1 phase arrest.  Induction of cell cycle arrest occurred in a p53-independent manner, through down-regulation of kRas.  Further studies are needed to determine the underlying mechanism of these effects and determine if growth inhibition occurs in vivo.