Determining the role of the RNA Binding Protein HuR in the RNA stability of antizyme and antizyme inhibitor

Open Access
Author:
Neiman, Margaret Mary
Area of Honors:
Biochemistry and Molecular Biology
Degree:
Bachelor of Science
Document Type:
Thesis
Thesis Supervisors:
  • Shannon Lyn Nowotarski, Thesis Supervisor
  • Jeanne Marie Rose, Honors Advisor
Keywords:
  • antizyme
  • antizyme inhibitor
  • HuR
  • non-melanoma skin cancer
  • RNA stability
  • RNA binding protein
Abstract:
Non-melanoma skin cancer (NMSC) is the most commonly diagnosed cancer in the United States. NMSC has two common types: basal cell carcinoma and squamous cell carcinoma, which can be caused by exposure to UV radiation. The polyamine pathway, particularly the upregulation of ornithine decarboxylase (ODC), has long been studied and correlated with tumorigenesis, especially in skin. Two endogenous regulators of ODC, antizyme (AZ) and antizyme inhibitor (AZI), have been implicated in tumorigenesis as well, although via poorly understood mechanisms. AZ binds to ODC and regulates ODC’s degradation by the 26S proteasome. AZI is able to bind to AZ to thus prevent ODC degradation. Previously, it was found that ODC was regulated by the RNA binding protein, Human Antigen R (HuR). In our current studies we plan to test whether AZ and AZI are also regulated by HuR in the NMSC mouse cell line model.