Industrial Optimization of the Production of Cargo Loaded PIV5 Virus-Like Particles
Open Access
Author:
Meyer, Nate
Area of Honors:
Veterinary and Biomedical Sciences
Degree:
Bachelor of Science
Document Type:
Thesis
Thesis Supervisors:
Anthony Paul Schmitt, Thesis Supervisor Pamela Hankey Giblin, Thesis Honors Advisor
Keywords:
Paramyxovirus Virus-like Particles VLPs VLP Protein Delivery Fermentation Biotechnology Scale-up Suspension Cells CHO 293 Transfection PEI Virus Virology PIV5 Cell Culture Optimization Western Blot Luminometer Delivery Efficiency VLP Production Matrix Nucleocapsid Glycoprotein Fusion Budding Nanotechnology Viral
Abstract:
A novel protein delivery system involving cargo loaded paramyxovirus-like particles is currently being studied. Despite showing promise as a biotech platform, production of these cargo loaded virus-like particles (VLPs) is costly. Currently, production relies on expensive transfection reagents and adherent mammalian cell lines, neither of which are practical in large scale production. In order for this promising technology to take the next step towards industrial or clinical use, new methods of production must be developed. In this study, evidence from pilot-scale experiments suggests the viability of large-scale production of cargo loaded PIV5 VLPs via the use of polyethylenimine (PEI) transfection reagents in the producer suspension cell line, Expi 293F. The VLPs produced with these new methods were quality tested in their ability to deliver proteins, and these delivery experiments confirmed VLP functionality.
