The expression of surface markers CD14 and CD33 on red blood cell progenitor cells.
Open Access
- Author:
- Cannata, Brigette
- Area of Honors:
- Immunology and Infectious Disease
- Degree:
- Bachelor of Science
- Document Type:
- Thesis
- Thesis Supervisors:
- Robert Paulson, Thesis Supervisor
Pamela Hankey Giblin, Thesis Honors Advisor - Keywords:
- Erythropoiesis
Stress erythropoiesis
Anemia
Cell marker
CD14
CD33 - Abstract:
- Stress erythropoiesis is an alternate pathway for erythrocyte development, triggered by periods of stress including anemia, oxygen poor in-utero development, and other conditions that lead to hypoxia. This pathway allows for the rapid production of red blood cells (RBCs) via the expansion and differentiation of stress erythroid progenitors (SEPs). SEPs express unique cell surface markers that differ from those of mature erythroid markers. Upon secretion of erythropoietin (EPO), SEPs are triggered to differentiate into stress BFU-Es. A strong understanding of the cell surface markers that differentiate SEPs from mature erythrocytes, as well as those that differentiate stress erythrocytes from steady state erythrocytes, is important for expanding research on erythropoiesis and for improving therapies for relates diseases, like anemia. The research presented in this thesis was conducted to investigate the expression of two distinct cell surface markers, CD14 and CD33, in a population of immature stress progenitors, Kit+Sca1+ cells. Data was also collected for the expression of these markers in more mature, Kit+Sca1- cells. The results show that the expression of these markers is dynamic, changing from cell expansion to cell differentiation. Overall, the unique expression patterns of CD14 and CD33 show a potential use for delineating SEPs from stress BFU-Es, although additional studies must be conducted to determine the relationship between these two cell markers.