Antibiotic resistance has become a large, global crisis in recent years due to the misuse and overuse of antibiotics. Because of this large increase in resistance, new methods of creating antibiotics and targeting bacteria must be created to continue preventing widespread disease. Trans-translation is a bacteria-specific process allowing for the release of stalled ribosomes. Inhibition of the trans-translation process is a potential target for antibiotics. New trans-translation inhibitors have been found to alter the conformation of the ribosomal protein L27, suggesting a novel mechanism for specific trans-translation inhibition. Deletions were made at the N-terminus of L27 to determine if the protein interacted with the inhibitors after these deletions. Findings suggest changes in inhibitory concentrations among inhibitors, indicating different interactions with the L27 protein.
