FLUORESCENT ACTIVATED ACOUSTIC CELL SORTING STRATEGIES
Open Access
- Author:
- Zhao, Shixin
- Area of Honors:
- Engineering Science
- Degree:
- Bachelor of Science
- Document Type:
- Thesis
- Thesis Supervisors:
- Jun Huang, Thesis Supervisor
Dr. Gary L Gray, Thesis Honors Advisor
Judith A Todd Copley, Faculty Reader - Keywords:
- Acoustic Cell Sorter
Fluorescent Activated
High Throughput Rate
Flow cytometry - Abstract:
- The cell sorter has received more attentions in recent years. The successful cell sorting can benefit biological or biomedical engineering field a lot. Biomedical experiments need exact controlling of the cells or other biological factors use. Researchers always need to extract or separate specific cell type from a group of mixtures. The traditional flow cytometry has been taken those jobs for decades. However, the traditional cell sorters are so expensive and may cause the damage of subjects. Acoustofluidic, a newly developing sorting method, has been brought onto the table with the advantage of high biocompatibility, cheap components, and portable size. In my senior project, I have been worked closely with Liqiang Ren on acoustic wave cell sorter in the acoustofluidics group of Dr. Huang in Pennsylvania State University. We have been working on improving the throughput rate of fluorescent acoustic wave cell sorter. Using the focused interdigital transducers and fluorescent recognizing system, I have fabricated the acoustic wave cell sorting device with the throughput rate as high as 720 events per second and increased the purity of the living cells from 20 percent to 78 percent.