The Effect of Selected Transcription Factors on Inhibiting In Vitro And In Vivo Hepatocellular Carcinoma Development

Open Access
Lin, John Lizhong
Area of Honors:
Bachelor of Science
Document Type:
Thesis Supervisors:
  • Gong Chen, Thesis Supervisor
  • Bernhard Luscher, Honors Advisor
  • liver cancer
  • HCC
  • Hepatocellular Carcinoma
  • HepG2
  • Hep3B
  • AFP
  • Ki67
  • Gene Therapy
  • Viral Infection
  • Transfection
  • Cancer Treatment
Liver cancer is a deadly disease that still has no effective therapy, despite many years of research. In this study, we have tested several transcription factors to see if they have any effects on HepG2 and Hep3B cancer cell lines, which are derived from patients with hepatocellular carcinoma (HCC). Both in vitro and in vivo experiments were performed using three or four transcription factors that were induced in these cell lines using adeno-associated virus 2 (AAV2) or lentivirus (LV) infection or transfection. The aim was to see if the overexpression of a few key factors in the cancer cell lines could stop cancer cell proliferation and downregulate liver cancer biomarkers. The results indicated that in HepG2 cells grown in vitro AAV2-mediated delivery of our transcription factors has low infection efficiency. But some transcription factors could significantly downregulate proliferation when compared to control, cells infected with empty viral vectors. Similar results could be seen in the Hep3B cell line. In addition, lentiviral-mediated expression of the same transcription factors supported these data. However, the results for transfection and in vivo studies did not demonstrate these same effects as clearly, largely due to low infection efficiency, which we are currently focusing to solve. In summary, my data suggests that overexpressing some of the transcription factors can significantly downregulate proliferation and cancer biomarkers through viral infection in vitro while my transfection and in vivo data may need additional experimentation. NOTE: The names of the transcription factors used are not specified in the thesis due to conflict with a patent application process and manuscript publication.